Angiogenesis is a fundamental process in the development and progression of many tumor and ischemic cardiovascular pathologies. Commonly used angiogenesis assays are two-dimensional, do not take into account the variability of microenvironments encountered and are unable to analyze microvascular perfusion. Using microfabricated and micropatterned patches, we have developed an interface enabling high-resolution 3D imaging and quantitative 3D analysis of angiogenesis under controlled microenvironmental conditions. The interface is integrated into a microfluidic chip for flow control. The characterization of angiogenesis, anastomosis and perfusion in this tool provides access to numerous tests in basic microvascular research and biomedical applications aimed at characterizing the impact of pharmacological agents as well as patient cells or sera.
Collaborators
Laurent Muller
INSERM Researcher, Center for Interdisciplinary Research in Biology (CIRB), Collège de France
Savitashva Shringi
PhD candidate, PASTEUR lab, Chemistry Department, ENS / CIRB, Collège de France
Leire Goni-Ciaurriz
Postdoctoral researcher, PASTEUR lab, Chemistry Department, ENS / CIRB, Collège de France
Carole Aimé 